Chromatography

Chromatography is one of several separation techniques defined as differential migration from a narrow initial zone. Electrophoresis is another member of this group. In this case, the driving force is an electric field, which exerts different forces on solutes of different ionic charge. The resistive force is the viscosity of the non-flowing solvent. The combination of these forces yields ion mobilities peculiar to each solute. Chromatography has numerous applications in biological and chemical fields. It is widely used in biochemical research for the separation and identification of chemical compounds of biological origin.In the petroleum industry the technique is employed to analyze complex mixtures of hydrocarbons.As a separation method, chromatography has a number of advantages over older techniques—crystallization, solvent extraction, and distillation, for example. It is capable of separating all the components of a multicomponent chemical mixture without requiring an extensive foreknowledge of the identity, number, or relative amounts of the substances present. It is versatile in that it can deal with molecular species ranging in size from viruses composed of millions of atoms to the smallest of all molecules—hydrogen—which contains only two; furthermore, it can be used with large or small amounts of material. Some forms of chromatography can detect substances present at the picogram (10−12 gram) level, thus making the method a superb trace analytical technique extensively used in the detection of chlorinated pesticides in biological materials and the environment, in forensic science, and in the detection of both therapeutic and abused drugs. Its resolving power is unequaled among separation methods.

  • Fundamentals of Chromatography
  • Types of Chromatography
  • Chromatography Software
  • Applications of Chromatography
  • Chromatography Solutions

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