Proteins and Peptide analysis in Mass Spectrometry

The MS-based detection of peptides and proteins in biological matrices can be divided in two main steps; they are sample clean-up and MS detection. The major challenges to detect growth promoting proteins and peptides are their generally low concentration, the complexity of the surrounding matrix, and the presence of endogenous proteins or isoforms. The complexity of the biological matrix requires highly specific clean-up procedures that are still compatible with the subsequent MS analysis. Common issues related to sample clean-up and MS detection approaches are, therefore, briefly summarized in the following section.

  • Protein Structural Analysis by Mass Spectrometry
  • Protein Therapeutics: Practical Characterization and Quantitation by Mass Spectrometry
  • Radical Peptides
  • Glycans and Glycoproteins in Mass Spectrometry
  • Protein phosphorylation and non-covalent interaction

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