Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 6th Global Congress on Mass Spectrometry Osaka, Japan.

Day 1 :

  • Fundamentals of Mass Spectrometry | Analytical Chemistry
Speaker
Biography:

Gongke Li obtained her PhD degree in analytical chemistry from Sun Yat-sen University of China in 1992. She then joined Sun Yat-sen University and became a professor since 2000. With expertise in chromatographic analysis and spectral analysis, her major research interests are focused on sample preparation techniques, analytical techniques for trace analysis of complex samples and coupling device for online analysis. She also studies both analytical- and preparative-scale separations methods for natural products. She has published more than 320 research papers in reputed journals, 14 authorized invention patents and 1 monograph. She is currently the associate editor of Journal Separation Science. 她2015年获中国化学会分析化学委员会首次颁发的中国女分析化学家奖She was awarded the Chinese women's analytical chemist in 2015. She was also named as one of the 2016 Power List and the Top 50 most influential women in the analytical sciences in the world (The Analytical Scientist’s 2016 Power List)

Abstract:

Abstract: Microporous organic polymers (MOPs) are generated by linkages of organic polymerizable monomer building blocks, providing high permanent porosity and excellent stability. Herein, we reported the first example of the application of organic building block based MOPs (OBB-MOPs) as efficient enrichment media for sample preparation. A novel multilayer inter-bridging strategy was proposed to fabricate OBB-MOP coatings on silica substrate with well-controlled thickness. Strong covalent bonds throughout the network and interlayer bridging improved the durability of the coating significantly. Outstanding chemical stability was observed. SNW-1 coating possessed microporous network structure constructed by conjugated and nitrogen-rich building blocks. Thus, the coating exhibited a superior enrichment performance of polycyclic aromatic hydrocarbons and volatile fatty acids (VFAs) over commercial coatings based on interactions including π-π affinity and acid-base interaction. This coating was combined with gas chromatography/mass spectrometry for the non-invasive analysis of VFAs from tea leaf and tobacco shred samples. The low detection limits of 0.014-0.026 μg/L were achieved with the RSDs between 4.3-9.0%. Good recoveries of the samples were obtained in the range of 90-129% and 77-118% with the corresponding RSDs of 2.6-9.3% and 1.9-10%, respectively.

Acknowledgements: The work were supported by the National Natural Science Foundation of China (Nos.21475153, 21675179 and 21605178), the Guangdong Provincial Natural Science Foundation of China (No. 2015A030311020), the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China (No.2015A030401036), and the Guangzhou Science and Technology Program of China (No.201604020165), respectively.

Yi Chen

Chineses Academy of Sciences, China

Title: LC-MS/MS of ultra-trace plant hormones in limited samples
Speaker
Biography:

Yi Chen has completed his PhD in 1990 and is now a full professor if the Institute, chairing Analytical Chemistry Department at University of CAS and leading a research group on chemical perturbation analysis of trace and ultra-trace bioactive substances in living things. He has published more than 270 papers and 3 books and owned 25 patents. He is serving as a deputy editor of Electrophoresis and editorial/advisory board members of 15 journals, and vice presidents of 5 Chinese academic societies including Professional Committee of Analytical MS in Chinese Chemical Society.

Abstract:

Bioactive substances often play their bio-functional roles at a very low concentration, for example, plants and insects may response to a minute variation of some special bio-chemicals such as hormones. This seriously challenges analytical methodology which is worsen in many cases by the unavailability of samples. In this presentation we are discussing a strategy to overcome the issues by use of high performance chromatography hyphenated with tandem mass spectrometry, on the topic of precise determination of plant hormones that exist in only a single tiny flower or even its floral organ. The related data will concern mainly with the distributions of a family of plant hormones, gibberellins (GAs), and their concentration, along the synthetic and metabolic pathways of GA1 and GA4 in different floral organs of one flower from a model plant arabidopsis thaliana. The flower has only a size even smaller than a drosophila melanogaster. The determination was enabled by use of new mass spectrometric techniques in combination with chemistry to sensitize the detection. This is validated to be an effective way at present to overcome the detection problem in ultra-trace analysis. The presented method is also extendable to the quantitative analysis of other bioactive organic acids and the study is discussed a bit more during the conference.

Speaker
Biography:

King-Chuen Lin is a Distinguished Professor of the Department of Chemistry at National Taiwan University and a Distinguished Research Fellow of National Science Council, Taiwan. He received his B.S. degree in Chemistry from National Taiwan University, Taiwan, his Ph.D. in Chemistry from Michigan State University, USA, and his postdoctoral career at Cornell University. His research interests are photodissociation and reaction dynamics in gas and condensed phases, atmospheric chemistry, and single molecule spectroscopy. He has published more than 172 related papers.

Abstract:

By using the time-of-flight mass spectrometer, we have provided insight into interrelation of the ionization and fragmentation mechanism of ketene. When a resonance-enhanced multiphoton ionization (REMPI) technique is applied via the Rydberg state, the (2+1) REMPI process leads to a direct photoionization of ketene under a low ionizing laser energy. When the (2+2) REMPI process dominates, the ketene ion is produced by the rovibrational autoionization of a super excited state.
The second application is to investigate competitive bond dissociation mechanisms for bromoacetyl chloride, 2- and 3-bromopropionyl chlorides following the transition 1 [n(O)®π* (C=O)] at 234-235 nm. The branching ratios of C-Br/C-Cl bond fission were evaluated to be 0.47, 0.24, and 0.098, respectively, by using (2+1) REMPI technique equipped with velocity ion imaging. The mechanisms for C-Cl and C-Br bond fission are discussed.

If time is allowed, we will report photodissociation dynamics of methyl formate HCOOCH3 at photolysis wavelengths from 225 to 255 nm. Ion imaging of CO and H are acquired. The translational energy distributions of CO comprise three channels of triple fragmentation, roaming and transition state (TS) processes, as photolysis wavelength is <248 nm. In contrast, the CO distribution is contributed by the roaming and the TS processes at >248 nm

Speaker
Biography:

Lin Zhang has completed her PhD at the age of 27 years from Shenyang Pharmaceutical University. During the doctoral period, she has been studied in Meiji Pharmaceutical University for one year and three month. She is the associated professor in College (Institute) of Integrative Medicine. She has published more than 20 papers in reputed journals and has been serving as an committee member of many branches of World Federation of Chinese Medicine Societies.

 

 

Abstract:

LC-MS as a technological mean, has been widely used in the field of clinical medicine and traditional Chinese medicine, including Quality control and pharmacokinetics study, take Zibu Piyin Recipe, Shuanghua Baihe tablets, Chaiqin Qingning capsule as an example; metabonomics and proteomics study, take Diabetes-Associated Cognitive Decline as an example. In this presentation, we hope to share our team’s work about Mass Spectrometry with everyone.

Speaker
Biography:

Dr. Wu completed his doctorate in analytical chemistry with the preparation of porous monolithic capillary columns and the separation of peptides in capillary electrochromatography at Dalian Institute of Chemical Physics (DICP), the Chinese Academy of Sciences (CAS), China, in 2002. After his study of high-voltage and high electroosmotic capillary electrochromatography at Max-Planck-Institute for Dynamics of Complex Technical Systems (Germany) in 2002-2003 as a post doctorate, he joined the School of Public Health at University of North Carolina at Chapel Hill (USA) in 2003-2006, where he pursued his research in bioanalytical chemistry regarding exposomics and lipidomics on PAHs and choline etc with mass spectrometry. In 2007, he went back to Dalian Institute of Chemical Physics (DICP), the Chinese Academy of Sciences (CAS), China, for the CAS Hundred Talent Program. Now, Dr. Wu is focusing on 1) method development of proteomics, peptidomics, lipidomics, and low-carbonomics etc. with multidimensional coupling of chromatography and mass spectrometry (LC/MS/MS) systems; 2) the multi-scale sample preparation of complex samples with advanced porous micro/nanomaterials; 3) the interactomics of material with biological molecules including protein corona, peptide corona, and lipid corona

Abstract:

Proteomics reveals the global drafts of proteins in biological systems, with the recent discovery and identification of 92% proteins encoded by human genome. The deeper insights of proteins in living systems call for the compliment with dynamic changes in localization, abundance, and interactions of proteins. Trials have been made to perform in situ and dynamic profiling of proteome/peptidome at the subcellular range by the combination of pre-fraction of subcellular components in vitro with isotope labeling at different time points and quantitative proteomics. Other than utilizing in vitro protein collection and labeling strategies, proteins obtained from physiological ambient without the chemical derivatizations such as in situ extraction with live cells are preferred for proteomic investigation containing high temporal and spatial resolution. 
To achieve the in situ and timing harvest of intracellular proteome and peptidome from a living system, the extraction probes should meet following essential requirements: 1) biocompatible with live cells; 2) suitable for both general or selective extraction of intracellular proteome/peptidome; and capatible to harvest compounds at 3) designated intracellular loci, and at 4) appointed time period. So far, the conventional proteome/peptidome sampling methods including micro extraction, electroporation could hardly satisfy all the above requirements. Nanoprobes, owning high biocompatibility and abilities for subcellular targeting and controllable intracellular transportation, hint promising potentials to extract cellular components from live cells in situ. In earlier attempts, heparin sulfate proteoglycans (HSPGs) antibody functionalized magnetic particles were employed to collect targeted endocytotic vesicles for proteomic analysis of HSPGs induced endocytosis,[5] methotrexate (MTX) linked dendrimers were applied in proteomic discovery of drug substrates from live cells,[6] and magnetic carbon nanotubes were used for harvest nucleic acid associated proteins in situ coupled with proteomic exploration[7]. However, nanocarriers for dynamic extraction of proteins are not satisfiable yet, since the proteins would be adsorbed by nanocarriers as soon as the cellular internalization. In this paper, a photo-switchable mesoporous silica nanocarrier (MSN with coumarin gates, MSNcg) was constructed as a general nano platform to collect cytosol polypeptides from live HeLa cells for the in situ and timing proteomic investigation.

Speaker
Biography:

Dr. Masashi Nojima, Ph.D.-engineering, now is a Junior Associate Professor of engineering, Research Institute of Science and Technology, Tokyo University of Science. He completed his doctorate in the micro-beam analysis in 2003. He has invented shave-off depth profiling by utilizing FIB micro-machining and SIMS. He applied the shave-off depth profiling for failure analysis of nano-devices: electro-chemical-migration phenomena in 2005-2008. In 2012, he has developed Ar gas cluster ion beam (GCIB) dynamic SIMS system for polymer depth profiling and imaging. From 2013, he has started to develop a new principle mass analyzer supported by JST-SENTAN program.

Abstract:

We have invented a new type of mass analyzer, based on a different principle from that of magnetic-sector, time-of-flight (TOF), or quadrupole mass separators. The new principle, which involves introducing a pair of rotating electric fields (REFs). Although earlier studies have already used a REF as the basis of mass separation, a single REF is seldom sufficient for separating ion trajectories based on the ion mass. Those studies also included TOF theory for REF based mass separation. In theory, the new mass analyzer with two REFs shows great promise for separating ion trajectories based on the ion mass. It features a compact ion optical system, which can be designed to fit within a polyethylene terephthalate bottle. The two REFs are generated by applying an AC voltage to a pair of octagonal electrodes optimized with a SIMIONTM simulator, in terms of both shape and interval. The REFs rotate with the same speed and axis, albeit with opposite phases.

We developed a prototype of this REFs mass analyzer using an optimized pair of octagonal electrodes and a focused-ion-beam (FIB) column. And demonstrated the potential to separate different masses simultaneously within continuous beam and also separate gigantic masses i.e. cluster ions, polymer ions, and proteins