9^th Global Summit on Mass Spectrometry October 16-17, 2020 Munich, Germany

Mass spectrometry (MS) has evolved to become a powerful analytical tool for both quantitative and qualitative applications, Applications of MS includes the ion and weights separation. The samples are generally introduced through a heated batch inlet, heated direct insertion probe, or gas chromatograph. Ionization (MS) technique has developed to be significant in the field of clinical laboratory for structural study or quantitative measurement of metabolites in a compound biological sample. TANDEM mass spectrometry applications are many, for elucidation of structure, determination of fragmentation mechanisms, determination of elementary compositions, applications to high-selectivity and high-sensitivity analysis, observation of ion–molecule reactions and thermochemical data analysis (kinetic method).

Mass spectrometry (MS) has evolved to become a powerful analytical tool for both quantitative and qualitative applications. The first mass spectrometer was developed in 19th century and since then it has developed from analyzing small inorganic molecules to biological macromolecules, almost with no mass limitations. Proteomics research gradually depends on MS technologies. The capability of mass spectrometry analyzing proteins and other biological extracts is due to the advances gained through the advancements in soft ionization techniques such as electrospray ionization and matrix-assisted laser desorption ionization can transform biomolecules into ions. ESI can effectively be interfaced with separation techniques enhancing its responsibility in the life and health sciences. MALDI, however has the advantage of producing individually charged ions of peptides and proteins, minimizing spectral complexity. Irrespective of the ionization source, the sensitivity of a mass spectrometer is related to the mass analyzer where ion separation takes place. Both quadrupole and time of flight mass analyzers are typically used, and they can be configured together as Q-tof tandem mass spectrometric instruments. Tandem (MS) (MS/MS), as the name signifies, is the consequence of performing two or more sequential separations of ions usually coupling two or more mass analyzers. Coupling a quadrupole and (TOF)resulted in the production of high-resolution mass spectrometers (Q-tof).

Mass spectrometry imaging is a method utilized in mass spectrometry to visualize the spatial distribution of compound for example biomarker, metabolites, peptides or proteins by their atomic masses. Although broadly utilized conventional techniques like radiochemistry and immunohistochemistry accomplish a similar objective as MSI, they are restricted in their capacities to investigate different examples on the double and can demonstrate to be missing if scientists don't have earlier information on the samples being analyzed. Radiology in the field of MSI are MALDI imaging and optional particle MS imaging. Imaging Mass Spectrometry is an innovation that consolidates progressed explanatory systems for the investigation of biomedical Chromatography with spatial devotion. A successful methodology for imaging natural examples along these lines uses Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS). Briefly, atoms of interest are inserted in an organic matrix that intensifies and aids the desorption and ionization of compounds on irradiation with an UV laser. The mass-to-charge proportion of the particles are estimated using a Tandem Mass Spectrometry over an arranged cluster of removed spots. Numerous analytes are estimated all the while, catching a portrayal or proof the natural condition of the particles in that example at an area on the tissue surface.

Along with hyphenated technologies such as (LC-MS) as well as (GC-MS), MS facilitates the establishment and advancement of high-throughput, qualitative and quantitative analytical methods used for drug discovery. In order to advances in LC and MS have enabled more efficient peak separation of complex mixtures employing multi – dimensional LC (2DLC) and more precise, improved resolution and ion mobility partition by MS. A selection of advanced LC-MS technologies applied to drug discovery chemistry, comprising compound ionization hyphenated systems, multidimensional systems, ion mobility spectrometry and HRMS for quantitative analysis and impurity detection.

Ionization techniques facilitate the examination of a variety of samples in their original state by Mass spectrometry and, are rapidly advancing in all fields where screening for the presence of various analytes in a chemical compound and data output fashion is desirable. innovation of atmospheric pressure ionization technique referred to as liquid matrix-assisted laser desorption electrospray ionization (LIQUID-MALDESI) to multiply charged ions by laser desorption from liquid samplesMatrix assisted laser desorption ionization.

Mass spectrometry is widely being applied to study biomolecules and the fastest developing field is the global analysis for identification and quantification of proteins, proteomics. Neuro-proteomics is a complicated method that has a long way to go in terms of profiling the whole neuronal proteome. It’s quite recent field that has many applications in therapy and science.

The use of mass spectroscopy in clinical laboratory has focused on newborn screening, steroid analysis, and drug abuse confirmation, it is not confined with present clinical applications and continues to expand. It is being used in almost every clinical field.

A wide range of contaminants have the potential to cause harm to humans and animals. They may be found in the air water soil and may come from the sources such as pesticides industrial waste landfill sites pharmaceutical drugs. Identification of these pollutants is challenging because of presence of wide range of potential components with varying chemical compositions. These contaminants require monitoring at very low concentration levels. These challenges call for advanced analytical techniques which are very sensitive and robust, fast, and cost effective. Mass spectrometry is renowned technique which can help in detecting environmental contaminants. With the advancements in techniques Mass spectrometry have improved applicability to environmental analysis.

Toxicology is a disciplinary study of poisons, designed to correlate the quantitative and qualitative interactions between poisons and their physiological and behavioral impacts on living system. Some important aspects of toxicology focus on elucidation of the mechanism of action of poison and enhancement of remedies and treatment plans for toxic effects.

Metabolomics and lipid omics are effective tool that aims to understand metabolites and lipids present in samples of biological origins. Variations in the species or amounts can be used to distinguish phenotypes and biological responses for diseases, genetic modifications, or nutritional and pharmacological treatments. This data lets scientists to understand how an organism works, or to know the underlying mechanism of a disease.

Along with the expanding use of mass spectrometry in laboratories with people not specifically trained in analytical chemistry there is a need for learning the analytical skills to maintain instrument performance and to get quality data output. The performance of a mass spectrometer will be severely compromised if it lacks the good vacuum in the ion transfer region of mass analyzer.  As the vacuum deteriorates it will become insufficient to maintain biomedical instrumentation in the operating mode. If the fore line pump is not well maintained, the oil may become so contaminated that the optimum pumping is no longer possible.  Primarily, gas transport and metabolism ballasting may clean the oil.  If the oil has become discolored, then it should be changed according to the pump manufacturers maintenance manually.

Mass spectroscopy is a powerful tool with many applications in pharmaceutical and biomedical field. The increase in sensitivity and resolution of instruments has opened a new dimension in analysis of pharmaceuticals.

The right software is the key to successful performance of MASS SPECTROMETERS. Mass spectrometry software has different options from routine to advanced packages for ensuring effective and efficient data handling and analysis of small molecules.

The coupling of two different techniques with the help of proper interface. mainly chromatographic techniques are combined with mass spectroscopic techniques. These techniques lead to better analysis of components and show more specificity and sensitivity.

Liquid chromatography-mass spectroscopy analysis of molecules from biofluids needs sensitive and robust assays. Due to the terribly complexed nature of the many biological samples, efficient sample preparation protocols  are required to get rid of unwanted elements and to selectively extract the compounds of interest, is a vital a part of every bioanalytical progress.
High-performance liquid natural process (HPLC) is  a separation technique that is used for the analysis of organic molecules and ions. HPLC relies on mechanisms of adsorption, partition and ion exchange, reckoning on the sort of stationary phase used. HPLC involves a solid stationary part, ordinarily packed within a stainless-steel column, and a liquid mobile part. Separation of the elements of a solution results from the differences within the relative distribution ratios of the solutes between the 2 phases. HPLC are often used to assess the purity and/or analyze the content of the many pharmaceutical bioprocessing substances. It also can be used to verify enantiomeric composition, using appropriately changed mobile phases or chiral stationary phases. Individual separation mechanisms of surface adsorption, partition and ion exchange not frequently occur in isolation since many principles act to particular degree at the same time.