Jing Quan Lim has completed his BSc from the Nanyang Technological University. He has experience in Pharmaceutical Testing, especially, in the areas of Western Medicines and Complementary Health Products Analysis. He is currently the Team Leader at the Laboratory’s Western Medicines team and plays a vital role for investigative samples
In this study, an LC-MS/MS with scheduled MRM and EPI method was developed for simultaneous determination of 40 drug substances with weight loss effect, including bisacodyl, phenolphthalein, sibutramine and its metabolites, etc. These drug substances may be adulterated in health supplements to promote significant weight loss effects. The samples were analyzed using a Q-Trap 5500 LC-MS/MS using a CORTECS UPLC C18 column (100 mmx2.1 mmx1.6 µm). Scheduled MRM was used as survey scan; MS2 spectra acquired in the EPI mode were used to perform library searching to increase the confidence of detection. Good limits of detection (LODs) were achieved using this methodology, where majority of the analytes were able to be detected at less than 10 ng/g levels.
Caihong Wang is pursuing her studies in the Pharmaceutical Analysis department of Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College as a PhD student. She has published three papers in Chinese Chemical Letters, Phytomedicine, Journal of Pharmaceutical and Biomedical Analysis and has been taking part in many reputed conferences.
Xiao-Xu-Ming decoction (XXMD) is a traditional Chinese formula and first recorded in “Bei Ji Qian Jin Yao Fang”. It has been widely used to treat stroke in China. The active fraction of XXMD (AF-XXMD) exhibits similar pharmacological effects to XXMD. In the present work, a new strategy of the rapid discovery and identification of AF-XXMD compounds was established using high-performance liquid chromatography with high resolution mass spectrometry (HPLC-HRMS) and the mass spectral tree similarity filter technique (MTSF). With the MTSF technique, structurally-related compounds were discovered and identified by comparing the similarity between their mass spectral trees and library compounds. A total of 3362 compounds were automatically detected by HPLC-HRMS, and a 68 compounds were finally identified in AF-XXMD. The identified compounds included 14 library compounds with authentic standards, 50 structurally-related compounds fished by the MTSF technique, and 4 structurally-unrelated compounds identified by the manual method. This study successfully applied the MTSF technology for the first time to discover and identify the components of Chinese prescription. The results demonstrated that MTSF technique should be useful to the discovery and identification of compounds in Chinese prescription. In addition, the MTSF technique is a promising technique for the targeted phytochemical separation in the future.