Huan-Tsung Chang
National Taiwan University, Taiwan
Title: Analysis of Biomolecules through Nanomaterials Based Mass Spectrometry
Biography
Biography: Huan-Tsung Chang
Abstract
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using nanomaterials as matrix has become a powerful technique for the analysis of biomolecules, including proteins, DNA, peptides, aminothiols, and carbohydrates. SALDI-MS has been developed to improve sensitivity and quantitation, with minimum problems associated with sweet spots. Several successful examples of the analysis of biomolecules through SALDI-MS will be discussed in my talk. SALDI-MS with HgTe nanostructures as matrix is useful for the detection of proteins such as IgG, protein G, and their complexes. SALDI-MS allows simultaneous detection of single- and double-stranded oligodeoxynucleotides, with limits of detection at the femtomole-to-picomole level and sample-to-sample intensity variation < 23%. SALDI-MS using gold nanoparticles (Au NPs) is sensitive for the detection of the digest of Cyt c in the HeLa cells treated with etoposide (a commercial drug) or carbon dots (potential drug).