Wenjie Cao
SABIC Technology Center, Saudi Arabia
Title: Complications of Adduct Ions on MRM at Different LC-MS/MS Ionization Techniques
Biography
Biography: Wenjie Cao
Abstract
For the triple quadrupole LC-MS/MS instrument, the primary purpose or the most significant feature is the highest sensitivity among almost all, if not all, of the LC-MS/MS instruments by doing the Multiple Reaction Monitoring (MRM) testing. The ionization of a chemical in LC-MS/MS could be done with different ionization modes. The common ones are Electron Spray Ionization (ESI), the Atmospheric Pressure Chemical Ionization (APCI), and the Atmospheric Pressure Photo Ionization (APPI). The species and amount of adduct ions produced at each mode are quite different. Some type of the adduct ions may complicate the MRM testing by decreasing the sensitivities while some other adduct ions may prevent any reliable MRM testing. This research is going to do investigation on the differences of the types of adduct ions produced, and the amount of adduct ions produced at different ionization techniques. Some examples will be presented to show how the adduct ions may complicate the MRM testing, and the best ionization mode for some type of the targeted chemicals.