Current Trends in Mass Spectrometry

Biography

Biography: Sherry A. Tanumihardjo

Abstract

Vitamin A is an essential nutrient important for vision, reproduction, immune function, and cellular differentiation. Vitamin A status assessment is not straightforward. Serum retinol (vitamin A) concentrations are homeostatically controlled over a broad range of liver vitamin A concentrations, which are how vitamin A status is defined. Moreover, infection and inflammation decrease circulating retinol concentrations further confounding assessment. Retinol isotope dilution (RID) methods using a variety of mass spectrometric techniques are gaining momentum on the continent of Africa. A recent study in Zambia used 13C-labelled retinyl acetate to assess total body vitamin A stores in preschool children. Surprisingly, a large percentage of the children were diagnosed with hypervitaminosis A (>1 mol retinol/g liver). This was supported by hypercarotenemia and slightly elevated retinyl ester concentrations. In fact, the children in two of the villages experienced hypercarotenodermia during mango season the following year. These findings were attributed to vitamin A adequate dietary intakes, five years of high-dose vitamin A supplements, and widespread use of vitamin A-fortified sugar. Many African countries are now adopting and mandating vitamin A-fortified foods. In order to ensure that neither deficiency nor hypervitaminosis prevails, countries will need to adopt sensitive RID mass spectrometric methods to assess total body vitamin A stores in carefully selected groups that represent all population strata that are exposed to multiple vitamin A programs. The International Atomic Energy Agency has recently invested in three gas chromatography-mass spectrometers in Africa to further support the use of RID methods on the continent using deuterium and 13C.