Biography
Biography: Sermin Tetik
Abstract
Mass Spectrometry (MS) has become the method of fields and applications, including proteomics and proteomic analysis. MS is now routinely used to identify and quantify known and unknown proteins in our labs as a fundamental tool. Its accuracy, flexibility and sensitivity have permissed new approachs in the biological area, characterization of biopharmaceutics and diagnostic criteria. MS has allowed it possible to analyze with of all is many types of mass analyzers including ESI, MALDI, FT-MS, ion trap, time-of-flight, quadrupole, etc. Mass analysis of protein and proteomic(s) products have ranged in mass between 50-300.000 daltons, in attomole through nanomole quantities. Sample preparation techniques for MS-analysis is a important step in the proteomics workflow. In the protein analysis sample preparation is the most variable and time consuming step. In proteomics laboratory sample preparation, instrumentation and software are all critical to success and high quality results. There is different sample preparation techniques because we have no standard method for preparing including type, complexity, source etc. Proper sample preparation means better results.