Current Trends in Mass Spectrometry

Biography

Biography: Kalluru Seshaiah

Abstract

A simple, sensitive and rugged quantitative method for the determination of Hydralazine in human serum by gas chromatography-tandem mass spectrometry (GC-MS/MS) has been developed and validated. Hydralazine (1-hydrazinylphthalazine) is a vasodilator used to treat severe hypertension, congestive heart failure, myocardial infarction and preeclampsia. The HPLC methods reported for assays of Hydralazine in plasma are complicated because of rapid cooling and or separation of plasma immediately after withdrawing each blood samples, and Donnell et al.,1 and Woodworth et al.,2 have reported about the instability of Hydralazine at physiological pH. In the present study, a simple, specific and selective method has been developed for the determination of Hydralazine from plasma samples using GC-MS-MS. The sample preparation technique, described here involves rapid processing of samples and also rugged according to commonly acceptable FDA guidelines. Chromatographic separation has been achieved by GC with MS detector in positive ionization mode. Helium was used as the carrier gas and DB 5ms column, (30m X 0.25mm, 0.25µm (J& W; DB 5ms) film thickness Capillary column coated with cross Linked Polymer was used) gave a good peak shape for both analyte and internal standard. The retention time of Hydralazine and 4-methyl Hydralazine (IS) were 12.99 and 14.17 minutes respectively. The total run time of 20.00 min for each sample was freezed upon. A simple liquid-liquid extraction (LLE) technique was employed for the sample preparation that provided a good recovery of both analyte and IS. The LLOQ was 10.000ng/mL Hydralazine hydrochloride. The precision and accuracy of analyte at LLOQ concentration was found to be 6.423 % and 88.093 %, respectively. The CV % was 3.21% and 3.81% and % accuracy was 97.82% and 100.373% for LQC and HQC respectively.