Current Trends in Mass Spectrometry

Biography

Biography: Peter Mikuš

Abstract

A new highly advanced multidimensional analytical approach for the ultra-trace determination of target chiral compounds in unpretreated complex real samples was developed in this work. The proposed analytical system provided high orthogonality due to on-line combination of three different methods (separation mechanisms), i.e. (i) isotachophoresis (ITP), (ii) chiral capillary zone electrophoresis (chiral CZE) and (iii) triple quadrupole mass spectrometry (QqQ MS). The ITP step, performed in a large bore capillary (800 m), was utilized for the effective sample pretreatment (preconcentration and matrix clean-up) in a large injection volume (1-10 L) enabling to obtain as low as ca. 80 pg.mL-1 limits of detection for the target enantiomers in urine matrices. In the chiral CZE step, the different chiral selectors (neutral, ionizable, and permanently charged cyclodextrins) and buffer systems were tested in terms of enantioselectivity and influence on the MS detection response. The performance parameters of the optimized ITP – chiral CZE-QqQ MS method were evaluated according to the FDA guidance for bioanalytical method validation. Successful validation and application (enantioselective monitoring of renally eliminated pheniramine and its metabolite in human urine) highlighted great potential of this chiral approach in advanced enantioselective biomedical applications.