Current Trends in Mass Spectrometry

Biography

Biography: Guo-An Luo

Abstract

Free fatty acids (FFAs) including omega-3 and omega-6 polyunsaturated fatty acids were major derived from arachidonic acid (AA) and eicosapentaenoic acid (EPA), which plays important roles in many physiological processes, especially inflammation. Assessment of eicosanoids is important for understanding their homeostatic and pathophysiological roles in inflammatory disease. A new analytical approach focus on polyunsaturated fatty acids metabolome was established using isotope dilution, solid phase extraction and 2D nano liquid chromatography-nanospray ionization-time of flight-mass spectrometry (2D nano LC-NSI-Q-TOF/MS). Within 35 min, 58 FFAs, including free fatty acids, prostaglandins, lipoxins, resolvins, leukotrienes, thromboxans, maresins, hydroxyeicosatetraenoic acids as well as epoxyeicosatrienoic acids were quantified along with seven corresponding isotope dilution internal standards. The limits of quantification were between 0.008 and 20 pg per injection for 1L. The efficiency of separation were perfect, all of the standards were separated well (Figure 1). The method validations for linearity, accuracy, precision, recovery were satisfied. Besides, this approach was performed to analyze both of targeted and non-targeted metabolomics studies in serum samples of women with polycystic ovarian syndrome (PCOS) and health women, the results indicated that the significant differences noted in the levels of heptadecanoic acid, mysristic acid, pentadecanoic acid, 9E, 11E-octadecadienoic acid, arachidolic acid, palmitic acid, oleic acid and stearic acid (p < 0.001) as well as palmitelaidic acid (p < 0.01) (Figure 1). Women with PCOS may have a unique metabolomic finger print and a definitive study is feasible. These findings may indicate that the PCOS were positive correlation between patient and health woman in fatty acids syntheses.