Claudia S. Maier
Oregon State University, USA
Title: Tandem mass spectrometry for the characterization of peptide adducts of electrophlic lipoxidation products
Biography
Biography: Claudia S. Maier
Abstract
We report on the characterization and quantification of peptide adducts of reactive lipid peroxidation products using tandem mass spectrometric approaches using advanced quadrupole time of flight mass spectrometry platforms. The post-translational modification (PTM) of proteins by electrophilic oxylipids is emerging as an important mechanism that contributes to the complexity of proteomes. Protein carbonyls are recognized indicators of diverse pathological conditions associated to redox inbalance and oxidative stress. We will discuss chemoselectives probes in conjunction with advanced tandem mass spectrometry approaches for the detection, characteization and quantification of this class of analytes. We will demonstrate the use of multiple tandem mass spectrometry approaches including travelling wave ion mobility-enhanced tandem mass spectrometry for characterizing of isomeric peptide adducts of electrophilic oxylipids. Overall, we provide an update on mass spectrometric methods for the in-depth analysis of protein carbonyls, recognized markers of oxidative stress related post-translational modifications.