Advanced Mass Spectrometry & Chromatography

Biography

Biography: Dr Khadija Shahid

Abstract

Pregabaline (GABA receptor) is an alkylated analogue of inhibitory neurotransmitter γ-amino-butyric acid. Release of several neurotransmitters are substantially reduced by pregabaline. It reduces excessive firing of neurons and hence controls seizers. Potential anticonvulsant activity of pregabaline is the modern antiepileptic drugs that target neuronal voltage-gated sodium, potassium and calcium channels. Pregabaline is used in anxiety disorder. Neither Pregabaline is not used in USA or EU for treating sleep disturbance or enhancing slow-wave sleep. Pregabaline reduces RLS (restless leg syndrome). Imine ligand obtained from different heterocyclic, monocyclic and aliphatic compounds are reported to possess cytotoxic, antiepileptic, anticancer, anti-tubercular, antimalarial, vasorelaxing, neuropathic pain, analgesic and fungicidal activities. Schiff base Reactions have significant role in the synthesis of novel and physiologically reactive compounds. Current research work is an effort to synthesize novel metal complexes of Fe, Zn, Cu and Sn with the imine ligand. The ligand is prepared by reacting 3-(aminomethyl)-5-methyl hexanoic acid with benzaldehyde. Addition of metals to compounds containing imines enhances their biological activity. The structure elucidation is confirmed by FTIR, ¹H NMR and Mass Spectrometry. Agar well diffusion and agar tube dilution methods are used for antibacterial and antifungal screening respectively. Antioxidant scavenging activity is also observed from ligand and their respective metal complexes. MTT calorimetric assay is performed against HeLa cell lines that confirms the anticancer activity of ligand and their metal complexes. The metal complexes exhibited better activity than ligand. The compounds can be screened for more biological activities on further studies.