Wang-Hsien Ding
National Central University, Taiwan
Title: Application of ion-paring injection-port derivatization GC-MS to detect phenolic endocrine disruptors in human urine
Biography
Biography: Wang-Hsien Ding
Abstract
Due to thesuperior separation,high distinguishingpower and availabilityin most routine laboratories,GC-MS systemisafrequently used technique fordetectingorganic micropollutants in environmental, biota and food samples.To improve the GC chromatographic separationfor hydroxylated (polar) analytes, derivatization is typically usedto increase thevolatilityand to improve sensitivity.However, off-line derivatization is laborious and time-consuming. Injection port derivatization (IPD), also known as on-line derivatization, means that the derivatization reaction occurs in the hot GC injection port. IPD simplifies the sample preparation procedure, avoids the usage of extra experimental apparatus, reduces the amount of derivatization reagents and organic solvents, shortens the derivatization time, and increases derivatization efficiency and ensures more accurate quantitation. This studydemonstrated the feasibility of applyingsolid-phase extraction(SPE)coupled withion-paring injection-port derivatization(IP-IPD) GC-MSto detect phenolic endocrine disruptors in human urinesamples. Three commonly and most concern phenolic endocrine disruptors: bisphenol A,4-nonylphenolsand 4-t-octylphenol,were employed in the method development and validation of this study.The parameters affectingSPE andIP-IPDweresystematicallyinvestigated.Accuracy and precision were evaluated,and thesuitabilityof the methodfor the determination thetracelevelsof the targetanalytesin human urinesamples was demonstrated.