Xuewu Zhang
South China University of Technology, China
Title: Proteomic analysis of anti-photoaging activity of the acetylated and amidated peptide GMCCSR
Biography
Biography: Xuewu Zhang
Abstract
In previous study, a hexapeptide with sequence of GMCCSR was identified from trpsin-digested whole protein of Spirulina platensis, and further study demonstrated that this peptide possessed antioxidant and anti-aging activities in vitro. In this study, the acetylated and amidated modification were employed to improve skin permeability of the peptide. The in vivo experiments showed that the acetylated and amidated peptide GMCCSR exerted good anti-photoaging effects on skin of mice by increasing the collagen production and activities of antioxidant enzymes SOD, GSH-Px and CAT. Subsequently, proteomic analysis using iTRAQ-based mass spectrometry technology was used to explore potential mechanisms. The results indicated that the acetylated and amidated peptide differemtially influenced the expression of 60 proteins. Among them, 33 proteins were up-regulated and 27 proteins were down-regulated. Based on bioinformatics analysis, the metabolic pathways and network related to skin photoaging were identified.