Shahzad Ahmad
Hamdard University, India
Title: Recent Applications of Nano-LC-MS in Molecular Toxicology
Biography
Biography: Shahzad Ahmad
Abstract
Endocrine disrupting chemicals (EDCs) are synthetic compounds used for diverse range of application and interfere with endocrine system through various ways. Accordingly, the endocrine disruption is one the focal area of toxicology. 2D nano liquid chromatography coupled with mass spectrometry (nano-LC-MS) recently attracted the notion of molecular toxicologists due its role in proteomic analysis. Proteomic analysis is the keen interest of molecular toxicologists to understand the toxicity mechanisms of EDCs and other toxicants. Enhanced sensitivity is the main advantage of 2D nano-LC-MS which facilitates the more concentrated bands of peptide in MS offering the determination of low abundant compound in limited sample. Molecular targets of EDCs and other toxicants include various biochemical indices such as proteins or enzymes, therefore the proteomic analysis on exposure to various EDCs helps to find the toxicity mechanisms of these toxicants. In our previous research, we virtually found the interactions of some EDCs with the biochemical indices of glucocorticoid biosynthetic pathway which is the main pathway of adrenal cortex, indicating the potential of EDCs to inhibit the various proteins of adrenal gland. The proteomic analysis of adrenal gland in response to EDCs by 2D nano-LC-MS may reveal the toxicity mechanism of EDCs and helps in risk assessments. Therefore, in the same way 2D nano-LC-MS shows the potential to become the powerful research tool in the field of molecular toxicology.