Tiejie Wang
Shenzhen Institute for Drug Control, China
Title: Simultaneous quantification of eight active flavonoids constituents in Gleditsiae Spina by high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry
Biography
Biography: Tiejie Wang
Abstract
Gleditsiae Spina (GS, Zaojiaoci in Chinese), the thorn of Gleditsia sinensis Lam., is used as an anti-inflammatory, anti-tumor and antibacterial drug for hundreds of years in China and Japan. It is known that the main active constituents is flavonoid, however, there has been no specialized study on quantification the active constituents. The study was aimed at evaluating the contents of active flavonoids in GS, and laying a foundation for its further researches. In this study, we developed a method using high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for determining 8 active flavonoids constituents: taxifolin, quercetin, quercitrin, rutin, isoquercitrin, dihydrofisetin, dihydrokaempferol, and eriodictyol in GS. Crude drugs of GS were extracted with 70% ethanol in ultrasound extraction. Chromatographic separation was performed on a Shim-pack C18 column (75 mm×2.0 mm, 2.2 μm) within 13 min. Gradient elution was applied using a mobile phase of 0.05% acetic acid/methanol with a flow rate of 0.20 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. The method had good linearity (R2>0.9982), variations in the intra- and inter-day precision of all analytes were below 2.2%, and the accuracy was evaluated by a recovery test within the range of 99.6–101.9%. The method successfully quantified the 8 compounds in 34 sample batches of Gleditsiae Spina, and will be provide a new quality evaluation method for Gleditsiae Spina.