Mass Spectrometry

2D chromatography – efficient way to increase peak capacity. It is important for HPLC-UV applications requiring baseline peaksseparation. In contrast, MS detection utilizes ions resolution by m/z and chromatographic (non-isobaric) peaks overlay is often in LC/MS/MS analysis. Typical benefits of 2D chromatography implementation in Applications of Mass Spectrometry are minimum of signalsuppression, low and stable background and excellent peak shape. Given presentation we will be focused on three case studies: 1.The robust and routine method of sorbitol analysis from human plasma by LC/MS/MS. 2. Non-digest liberation of biomarkers from plasma: a unique two-stage ultrafiltration approach combined with 2D chromatography and mass spectrometry analysis. 3. Human C-peptide analysis by 2D RP-RP LC/MS. These case studies demonstrate additional unique advantages of 2D/column switching applications in mass spectrometry beyond mentioned above signal suppression/matrix effects minimization, background and peak shape improvements. Addition of switching valve and extra HPLC pump may contribute drastic improvements in LC/MS analysis.