Mass spectroscopy in Chromatography and HPLC

Each component in a mixture is separated, identified, and quantified using the analytical chemistry technique of high-performance liquid chromatography (HPLC), originally known as high-pressure liquid chromatography. A column of solid adsorbent material is moved through a pressurized liquid solvent that contains the sample combination using pumps. Each component in the sample interacts slightly differently with the adsorbent material, leading to a range of flow rates for the various components and their separation as they exit the column.

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