Biography
Abstract
The renin- angiotensin system (RAS) has long been recognized as an important regulator of systemic blood pressure and electrocyte homeostasis. Our view of RAS has experienced remarkable change over the past decades- new enzymes and products as well as their local formation and action have been described. Tissue RAS plays an important role in development of various diseases. All components of classical RAS have been found in various type of cancer tissue. It has been demonstrated that some angiotensins peptides can contribute to development and progression of tumour. Ang II stimulates cell proliferation and angiogenesis (also Ang III and Ang IV). On the other hand, Ang- (1-7) plays opposite role to Ang II, showing antiproliferative effects and reducing fibrosis, angiogenesis, and tumour volume and weight. However, there is still the lack of studies on role and action of newer angiotensins like Ang-(1-12), which is an alternative substrat for Ang II formation. The aim of this study was to estimate the main pathways and characterize the main products of metabolism of angiotensinogen fragments (Ang-(1-14), Ang-(1-12 and Ang I) in cancer cells. Using an LC/MS/MS method, we assessed the ability of breast cancer cells (MCF7, MDA-MB-231 and T47D) to produce main active angiotensins peptides. Better understanding of RAS role in cancer may represent a new approach for cancer prevention and treatment.
Biography
Abstract
Diagnosis of disease biomarkers like cancer, diabetes, etc. have been conducted by performing mass spectrometry techniques on human tear fluid. Apart from this, tear proteomics and lipidomics have been studied extensively by researchers worldwide for identification of disease biomarkers pertaining to the ocular surface disorders. One prime example of such disease is dry eye, where by applying mass spectrometry techniques researchers have shown absence or presence of certain proteins and lipids characterizing the disease. But in this study, we have shown that apart from the inter-individual change between diseased state and control, there are differences intra-individually both at the proteomics and lipidomics level. The results obtained indicate that there are changes in left and right eye proteomics and lipidomics profile with an individual. This study in future could help in diagnosis of ocular disorders pertaining to a particular eye as opposed to the general notions adapted by ophthalmologists that if one is in a diseased state the other one is also prone to be leading to that state. The reason for this difference may be of neurobiological origin which needs more detailed future study.